Multiple buttons pspps11/2/2022 NO treatment also delayed cellulose synthesis by increasing cellulase activity. NO treatment delayed softening by maintaining the integrity of the cell wall and inhibiting the degradation of protopectin and the expressions of pectin methylesterase and polygalacturonase. Without NO, fresh weight and firmness decreased quickly, while the cellulose and lignin contents increased sharply during storage. Here, we examined the effect of NO on the storage of water bamboo shoots at 4℃ for 28 days. Nitric oxide (NO) has been used to extend the postharvest life of several other vegetables. Water bamboo shoots quickly deteriorate after harvest as a result of rapid lignification and softening. Our results suggest the structure and functional properties of the films are closely related to the number of hydroxyl substituents at B-ring of flavonols. The release behavior of flavonols in different food stimulants greatly affected the antioxidant activity of the films. Kaempferol was easily released into fatty food stimulant, while myricetin was easily released into aqueous food stimulant. Notably, different films presented distinct flavonol release behaviors in fatty and aqueous food stimulants. Among all the films, the film containing myricetin showed the highest water vapor and oxygen barrier abilities and mechanical properties. The addition of flavonols reduced the water vapor permeability, oxygen permeability and UV–vis light transmittance of the films, whereas elevated the tensile strength, elongation at break, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and antimicrobial activity of the films. Results showed the incorporation of flavonols produced dense inner microstructure by establishing intermolecular hydrogen bonding interactions with film matrix. In this study, kaempferol, quercetin and myricetin were individually mixed with chitosan (CS) based film matrix to develop active packaging films. Kaempferol, quercetin and myricetin are typical flavonols that feature different number of hydroxyl substituents at B-ring.
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